February 1998

In This Issue:
        Higgins Cartridges in Disease Research
        0.5mm I.D. Columns Now Available

HAIPEEK Cartridge Columns and Holders

Disease Research
Micro-Preparative HPLC with Higgins Analytical Cartridge Columns help researchers understand the immune response of living cells:

An investigation to understand the genetic control of the immune response systems in organisms involves the extraction, isolation, and identification of more than 10,000 peptides. These distinct peptides are bound to a major histocompatibility complex (MHC) molecule on tumor cells.

Extraction of approximately 5x1010 cells yield several nanomoles of a very complex peptide mixture. Several rounds of RP-HPLC with different organic modifiers on a 40x2.1mm PEEK cartridge column (HAISIL 300 C18 5µm, P/N: HK-0421-W185) are performed to fractionate the mixture into individual components.

The chromatograms below illustrate how this technique is being used to narrow the search for the one peptide that marks a particular tumor cell as being diseased enabling it to be recognized by the immune cells.

Short and efficient HAIPEEK Cartridge Columns enable fast 40sec fraction collection. The stable HAISIL 300 C18 phase has proven batch-to-batch reproducibility and its selectivity can be varied with modifiers like HFBA and TFA to optimize the separation.

Check out http://www.mayo.edu/education/mgs/faculty/wettstei.htm   for additional information about related immunogenitic research.

Higgins Analytical is grateful to Mark Ross and Argonex, Inc., Charlottesville, VA for permission to us to share this interesting work.

An Expanded Microbore Offering
The peptide applications illustrated in  HAINEWS Vol. 5 and the myoglobin tryptic digest below, typify some of the enthusiastic reports we have received about our 1.0mm columns that were launched at HPLC '97. At Pittcon '98 this year, we featured an expanded microbore column offering that includes 0.5, 0.8, and 1.0mm I.D. Targa C18, HAISIL HL, HAISIL 100 C18, and HAISIL 300 C18 columns.

Ordering Information for 0.5 - 1.0mm I.D. Columns
Call 650-988-8930 or 800-988-6889 for pricing

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© 1998 Higgins Analytical, Inc (19Aug98)