HAISIL 300 Wide Pore Columns:  Higgins Analytical's HAISIL 300 C8 and C18 columns are popular alternatives for biomolecule analysis where larger pore diameters often enhance separations. High efficiencies, long column life, and extremely symmetrical peaks are characteristics of both the 4.6 and 2.1mm I.D. formats.

The Role of Column Length in Gradient Separations:  Changes in column length have a direct and linear effect on theoretical plates and analysis time in isocratic separations. Column length plays a less important role, however, in gradient techniques. The three chromatograms below illustrate how well packed short HPLC columns yield very similar peptide separations under identical conditions. For this study, the 2.1mm I.D. HAISIL 300 C18 5µm columns ranged from 250 to 100mm in length. While solvent and analysis time savings may be minimal, column cost savings can be significant when using as short a column as possible.

Higgins Analytical's reputation for manufacturing efficient and robust columns now ranges from 1mm to 20mm internal diameter. Algo, meaning "a sharp needle," appropriately describes the peaks obtained from Higgins Analytical's high performance 1mm I.D. HPLC columns. We're so confident about our Algo Series HAISIL 300 C18 5µm performance, we challenge anyone to manufacture a better one.

Depending upon the type of information a protein chemist is seeking, the HPLC analysis of a complex digest can take 1 to 2 hours. The following link is a high resolution tryptic digest map of BSA on a 250x2.1mm HAISIL 300 C18 5µm column indicates peak capacities in excess of 100. Small proteins, on the other hand can yield fewer fragments, and if only a "fingerprint" of a digest is required, high peak capacity columns are a waste of time.

The link below illustrates a very fast peptide map of cytochrome-C. The information that can be gleaned from this five minute profile is more than adequate for many analytical requirements. The robust 50x4.6mm HAISIL 300 C18 column provides many runs without performance loss even under the aggressive 6ml/min flow conditions used in this example.

Most pharmaceutical QC laboratories are faced with rapidly increasing demands for high throughput capability. A good example of one laboratory's approach to addressing a large number of samples for analysis on a daily basis is summarized below. This novel drug delivery system contains a small protein and other components that span an order of magnitude in molecular weight. All the compounds of interest were easily resolved in a single analysis with a fast linear gradient on a HAISIL 300 C18 5µm column.

Other HAISIL 300 Chromatograms:

HAISIL 300
Part Numbers

The following HAIPEEK and HAICART Guard Cartridges are compatible with HAIPEEK guard holders and the PE Brownlee NewGuard™ Holder.

The following HAIPEEK and HAICART Analytical Cartridges are compatible with HAIPEEK   holders and  PE Brownlee MPLC™ Holders.

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